Using the Bioruptor® for shotgun proteomics

Posted on February 20, 2017February 20, 2017 by Emilie Chabot

Mass spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team[…]

Long read sequencing: Complete genome of the hospital-acquired strain Staphylococcus aureus Tager 104

Posted on February 20, 2017 by Emilie Chabot

Staphylococcus aureus infections range in severity due to mobile genetic elements (MGE) and single nucleotide polymorphisms. The understanding of the evolution of these dangerous pathogens requires the definition of reference strains. Richard W Davis and colleagues published a method to construct and validate the assembly of the 2.82Mb S. aureus genomes. Authors used a previously uncharacterized strain isolated from a[…]