Researchers here provide evidence that SPOC1 overexpression severely impairs HCMV replication by repressing the initiation of viral immediate early (IE) gene expression. SPOC1-depleted primary human fibroblasts displayed augmented initiation of viral IE gene expression. This occurs in a MOI-dependent manner, a defining hallmark of intrinsic immunity. Interestingly, repression requires the presence of high SPOC1 levels at[…]
New application using the Bioruptor for DNA shearing before library preparation: In this study, researchers conducted a reciprocal transplant trial, a common garden trial, and a whole‐genome based demography analysis to understand how selection, migration and drift influence local adaptation in Arabidopsis lyrata. In a three‐year field experiment, they showed evidence of local adaptation at the level of hierarchical[…]
In this study, researchers performed in-depth virological and cell biological studies and discovered that an inner nuclear membrane protein, SUN2, is a novel chromatin reassembly factor that maintains repressive chromatin and thus modulates HIV-1 transcription and latency. Targeting SUN2 may lead to new strategies for an HIV cure. The well-cited Bioruptor was used to[…]
Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene[…]