DNA shearing is important step for next-generation sequencing library preparation, and reproducibility and efficiency of shearing are key factors. Nataliia Gralievska and Helle Ulrich at the Institute of Molecular Biology in Germany show that the Bioruptor®Pico is the best system to shear S. cerevisiae DNA. Since this yeast has smaller amounts of DNA per cell and a cell wall, the conditions they use for the shearing are different than for mammalian cells. They conclude that the Bioruptor® Pico provides a simple, reproducible and straightforward method for yeast DNA shearing to obtain small fragment library for Next-Generation Sequencing.Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS.
Read this application note from Nataliia Gralievska and Helle Ulrich Institute of Molecular Biology, Mainz, Germany
