Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene[…]
For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here researchers report a method revealing widespread and abundant protein histidine phosphorylation in Escherichia coli. They generated an extensive E. coli phosphoproteome data set, in which a remarkably high percentage of phosphorylation sites are phosphohistidine sites. This resource should help enable a[…]
Mass spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team[…]
The application of mass spectrometry for the analysis of protein complexes can be used in parallel with chromatin immunoprecipitation–sequencing experiments to provide information on both the cistrome and interactome for a given protein. Hisham Mohammed and colleagues developed the Rapid Immunoprecipitation Mass spectrometry of Endogenous protein (RIME) approach, a rapid and robust method to study chromatin and[…]