New application using the Bioruptor for DNA shearing before library preparation:
In this study, researchers conducted a reciprocal transplant trial, a common garden trial, and a whole‐genome based demography analysis to understand how selection, migration and drift influence local adaptation in Arabidopsis lyrata. In a three‐year field experiment, they showed evidence of local adaptation at the level of hierarchical multi‐year fitness, attesting to the strength of differential selection. In the lowland habitat, local superiority was associated with greater fecundity, while viability accounted for fitness differences in the alpine habitat. They also demonstrate that flowering time differentiation has contributed to adaptive divergence between these locally adapted populations. The results show that despite the estimated potential of gene flow and drift to hinder differentiation, selection among these A. lyrata populations has resulted in local adaptation. The Bioruptor Pico was an instrumental part of the sequencing workflow in this study, allowing optimal DNA fragment sizes to be generated for library prep. Read more.