New article that highlights the power of the Bioruptor in DNA shearing for NGS – Phylogenomic evidence for a recent and rapid radiation of lizards in the Patagonian Liolaemus fitzingerii species group

Rapid evolutionary radiations are difficult to resolve because divergence events are synchronous and gene flow among nascent species can be high. Large datasets composed of sequence loci from across the genome can potentially help resolve some of these difficult phylogenetic problems.  Researchers in this study chose a test case using the Liolaemus fitzingerii  lizards, which includes twelve species that are broadly distributed in Argentinean Patagonia. The researchers generated a sequence capture dataset for 28 ingroup individuals of 580 nuclear loci, alongside a mitogenomic dataset, to infer phylogenetic relationships among species in this group.   The interspecific relationships based on nuclear DNA were weakly supported and NDNA and mtDNA phylogenetic relationships were in conflict. Formal tests provided ambiguous support for hybridization. Lastly, species in the Liolaemus fitzingerii group constituted a recent and rapid radiation. The Bioruptor Pico was used to shear genomic DNA in this study prior to library preparation for sequencing.

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