In this study, researchers show that establishment of CD4CTX-specific transcriptional and epigenetic programs occurred in a stepwise fashion along the Th1-differentiation pathway. Activation of naive CD4 T cells in presence of Th1 polarizing cytokines led to the acquisition of perforin-dependent cytotoxic activity. This process was dependent on the Th1 transcription factor Runx3 and was limited by the sustained expression of ThPOK. This work elucidates the molecular program of human CD4CTX T cells and identifies potential targets for immunotherapy against viral infections and cancer. The well-cited Bioruptor Pico was essential to shear chromatin optimally prior to ChIP in this study. In addition, Diagenode’s ChIP-validated antibodies were used for chromatin immunoprecipitation.
