In this study, researchers further investigated the molecular mechanisms behind resistance to radiotherapy. They further investigated β1-integrin which was previously linked to cancer cell survival after irraditation. Among several findings, ectopic β1-integrin expression in S1 cells reduced RING1 levels and increased Rad51 accumulation. In contrast, β1-integrin depletion in T4-2 cells significantly increased RING1 protein levels and potentiated Rad51 ubiquitination. These data suggest for the first time that elevated levels of β1-integrin can increase tumor cell radioresistance by decreasing Rad51 degradation through a RING1-mediated proteasomal pathway. The well-cited Bioruptor Pico was used to optimally shear chromatin prior to ChIP in this study.
Read more.