Tag: DNA shearing

Local adaptation and ecological differentiation under selection, migration and drift in Arabidopsis lyrata

Posted on by Emilie Chabot

New application using the Bioruptor for DNA shearing before library preparation: In this study, researchers conducted a reciprocal transplant trial, a common garden trial, and a whole‐genome based demography analysis to understand how selection, migration and drift influence local adaptation in Arabidopsis lyrata. In a three‐year field experiment, they showed evidence of local adaptation at the level of hierarchical[…]

New publication! Application of next-generation sequencing to characterize novel mutations in clarithromycin-susceptible Helicobacter pylori strains with A2143G of 23S rRNA gene

Posted on by Emilie Chabot

Clarithromycin (CLR) resistance has become a predominant factor for treatment failure of H. pylori eradication. Although the molecular mechanism of CLR resistance has been clearly understood in H. pylori, it is lack of evidence of other genes involved in drug resistance. Furthermore, the molecular mechanism of phenotype susceptible to CLR while genotype of 23S rRNA is mutant with[…]

New article that highlights the power of the Bioruptor in DNA shearing for NGS – Phylogenomic evidence for a recent and rapid radiation of lizards in the Patagonian Liolaemus fitzingerii species group

Posted on by Emilie Chabot

Rapid evolutionary radiations are difficult to resolve because divergence events are synchronous and gene flow among nascent species can be high. Large datasets composed of sequence loci from across the genome can potentially help resolve some of these difficult phylogenetic problems.  Researchers in this study chose a test case using the Liolaemus fitzingerii  lizards, which includes twelve[…]