Long read sequencing: Complete genome of the hospital-acquired strain Staphylococcus aureus Tager 104

Staphylococcus aureus infections range in severity due to mobile genetic elements (MGE) and single nucleotide polymorphisms. The understanding of the evolution of these dangerous pathogens requires the definition of reference strains. Richard W Davis and colleagues published a method to construct and validate the assembly of the 2.82Mb S. aureus genomes. Authors used a previously uncharacterized strain isolated from a[…]

Capture d’écran 2016-07-05 à 15.18.19

Best shearing of yeast genomic DNA for NGS

DNA shearing is important step for next-generation sequencing library preparation, and reproducibility and efficiency of shearing are key factors. Nataliia Gralievska and Helle Ulrich at the Institute of Molecular Biology in Germany show that the Bioruptor®Pico is the best system to shear S. cerevisiae DNA. Since this yeast has smaller amounts of DNA per cell and a[…]